Title Medicament/Irrigant Selection and Concentration are Important to the Survival of SCAP and the Success of Regenerative Endodontic Treatment
Clinical Question In regenerative endodontic treatment, does Medicament/irrigant type and/or concentration influence SCAP viability?
Clinical Bottom Line It appears clinically important that in regenerative endodontic treatment medicaments/irrigants must be used at concentrations that are bactericidal while having minimal effects on SCAP viability.
Best Evidence  
PubMed ID Author / Year Patient Group Study type
(level of evidence)
22980180Ruparel/2012Stem cells of the apical papilla; 5 total groups; n=6/group.Laboratory study
Key resultsAntibiotic concentrations between 1 and 6mg/ml have a significant detrimental effect on SCAP survival (LD50). Lower concentrations of antibiotics as well as Ca(OH)2 at all tested concentrations are conducive to SCAP survival and proliferation (no significant effect on SCAP survival; p<.05)
24331991Martin/2014Stem cells of the apical papilla; 10 total groups; n=9/group.Laboratory study
Key resultsDentin conditioning with 1.5% NaOCl promoted a 40% and 300% greater rate of survival and differentiation of SCAPs than 3% and 6% NaOCl respectively when followed by 17% EDTA. (p<.05)
Evidence Search regenerative endodontics concentration
Comments on
The Evidence
Although the included studies are In vitro studies, they are valid and clinically relevant in that they investigate SCAP survivability and highlight the influence that medicament/irrigant type and concentration have on SCAP viability.
Applicability Promoting SCAP survival is critical to the success of regenerative endodontics. Clinicians must be aware of factors which promote SCAP survival and this success of regenerative endodontics including the type and concentration of selected medicaments and irritants.
Specialty (Endodontics)
Keywords regenerative endodontics, medicament, irrigant, concentration, stem cells, SCAP
ID# 2772
Date of submission 11/26/2014
E-mail kunzkr@livemail.uthscsa.edu
Author Kevin R. Kunz, DDS
Co-author(s) e-mail
Faculty mentor James Ball, DDS
Faculty mentor e-mail ballj@livemail.uthscsa.edu
Basic Science Rationale
(Mechanisms that may account for and/or explain the clinical question, i.e. is the answer to the clinical question consistent with basic biological, physical and/or behavioral science principles, laws and research?)
None available
Comments and Evidence-Based Updates on the CAT
None available